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Shina, C., Unal, C., Eichinger, L., Muller-Taubenberger, A., Schleicher, M., Steinert, M., Noegel, A., (2010) ' A Coronin7 homolog with functions in actin-driven processes. ' J Biol Chem 285 9249-61

 

Abstract:Dictyostelium discoideum Coronin7 (DdCRN7) together with human Coronin7 (CRN7) and Pod-1 of Drosophila melanogaster and Caenorhabditis elegans belong to the coronin family of WD-repeat domain-containing proteins. Coronin7 proteins are characterized by two WD-repeat domains that presumably fold into two beta-propeller structures. DdCRN7 shares highest homology with human CRN7, a protein with roles in membrane trafficking. DdCRN7 is present in the cytosol and accumulates in cell surface projections during movement and phago- and pinocytosis. Cells lacking CRN7 have altered chemotaxis and phagocytosis. Furthermore, loss of CRN7 affects the infection process by the pathogen Legionella pneumophila and allows a more efficient internalization of bacteria. To provide a mechanism for CNR7 action, we studied actin-related aspects. We could show that CRN7 binds directly to F-actin and protects actin filaments from depolymerization. CRN7 also associated with F-actin in vivo. It was present in the Triton X-100-insoluble cytoskeleton, colocalized with F-actin, and its distribution was sensitive to drugs affecting the actin cytoskeleton. We propose that the CRN7 role in chemotaxis and phagocytosis is through its effect on the actin cytoskeleton.
Status:	Published	Type:	Journal article	Source:	PUBMED	PubMed ID:	20071332

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	Genes addressed in this paper
	corB
Topics in this paper
Mammalian Gene Related	X
Adhesion	X
Protein Functional Domain	X
Cytoskeleton	X
Strains/Constructs	X
Cellular Location	X
Synonym	X
Function/Process	X
Chemotaxis/Motility	X
Endocytosis	X
RNA Levels and Processing	X
Protein-Protein Interactions	X
Regulatory Role	X
Mutants/Phenotypes	X
Growth	X